bioeng_admin
2005-12-14 21:28:41
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아래와 같이 세미나가 개최되오니, 관심있는 분들의
많은 관심 부탁드립니다.
제목: A novel FRET-based optical fiber biosensor for rapid and real-time detection of Salmonella Typhimurium
연사: Dr. Sungho Ko
소속: Bio&MEMS Team, New Technology/Analysis Dept.
National NanoFab Center (NNFC)
일시: 2005. 12. 14 (Wed) 15:10-16:00
장소: Room 220, Chung Moon Soul Building (E16)
Salmonella contamination in foods results in not only foodborne outbreaks, but also a large economical burden for the industry due to product recalls. As a result, a custom-built fiber optic benchtop biosensor utilizing the principle of fluorescence resonance energy transfer (FRET) was developed for the detection of Salmonella Typhimurium in ground pork samples. The FRET in this study is a result of the conformational change in the 3D structure of the antibody as it binds to the target antigen.
Labeled antibody-Protein G complexes were formed via the incubation of anti-Salmonella antibodies labeled with FRET donor fluorophores (Alexa Fluor 546) and the protein G (PG) labeled with FRET acceptor fluorophores (Alexa Fluor 594). The labeled antibodies-PG complexes were immobilized on decladded, tapered silica fiber cores using the silanization method to form the probe sensing region. Evanescent wave sensing was utilized.
The fiber probes were tested in a phosphate buffered solution to demonstrate the feasibility of the biosensor for detecting S. Typhimurium and to determine the optimal packing density of the labeled antibody-PG complexes on the surface of fibers. Different packing densities ranging from 0.01 mg/ml to 0.066 mg/ml were examined. It was determined that a packing density of 0.033 mg/ml produced the highest energy transfer and the lowest limit of detection of 103 cells/ml. The fiber probes were then placed in homogenized pork samples inoculated with S. Typhimurium for 5 minutes. The limit of detection was 105 CFU/g. The specificity of the proposed system was checked against Listeria monocytogenes, and it was not detected in this system.
These results show that the FRET-based fiber optic biosensor can become a useful analytical tool for rapid and real-time detection of S. Typhimurium in food samples.
많은 관심 부탁드립니다.
제목: A novel FRET-based optical fiber biosensor for rapid and real-time detection of Salmonella Typhimurium
연사: Dr. Sungho Ko
소속: Bio&MEMS Team, New Technology/Analysis Dept.
National NanoFab Center (NNFC)
일시: 2005. 12. 14 (Wed) 15:10-16:00
장소: Room 220, Chung Moon Soul Building (E16)
Salmonella contamination in foods results in not only foodborne outbreaks, but also a large economical burden for the industry due to product recalls. As a result, a custom-built fiber optic benchtop biosensor utilizing the principle of fluorescence resonance energy transfer (FRET) was developed for the detection of Salmonella Typhimurium in ground pork samples. The FRET in this study is a result of the conformational change in the 3D structure of the antibody as it binds to the target antigen.
Labeled antibody-Protein G complexes were formed via the incubation of anti-Salmonella antibodies labeled with FRET donor fluorophores (Alexa Fluor 546) and the protein G (PG) labeled with FRET acceptor fluorophores (Alexa Fluor 594). The labeled antibodies-PG complexes were immobilized on decladded, tapered silica fiber cores using the silanization method to form the probe sensing region. Evanescent wave sensing was utilized.
The fiber probes were tested in a phosphate buffered solution to demonstrate the feasibility of the biosensor for detecting S. Typhimurium and to determine the optimal packing density of the labeled antibody-PG complexes on the surface of fibers. Different packing densities ranging from 0.01 mg/ml to 0.066 mg/ml were examined. It was determined that a packing density of 0.033 mg/ml produced the highest energy transfer and the lowest limit of detection of 103 cells/ml. The fiber probes were then placed in homogenized pork samples inoculated with S. Typhimurium for 5 minutes. The limit of detection was 105 CFU/g. The specificity of the proposed system was checked against Listeria monocytogenes, and it was not detected in this system.
These results show that the FRET-based fiber optic biosensor can become a useful analytical tool for rapid and real-time detection of S. Typhimurium in food samples.
