1. Title: Exosome Biogenesis  & Protein Budding

2. Speaker: Stephen Gould

3. Date & time: 2017년 9월 12일  2:30 p.m.

4. Venue: 양분순빌딩 206호 계단강의실

5. Abstract

Current models of exosome biogenesis make discrete predictions about the intracellular trafficking pathways that are essential for a protein’s exosomal secretion.

For example, the hypothesis that exosomes arise solely by vesicular budding from the limiting membrane of the endosome predicts that exosomal marker proteins must first traffic to the endosome membrane prior to their vesicular secretion.

Furthermore, this ‘endosomal’ hypothesis of exosome biogenesis predicts that mislocalizing exosomal proteins to the plasma membrane should reduce and/or eliminate their vesicular secretion. Here we tested these and other predictions of this commonly accepted model. Using both mammalian and insect model systems, we find little empirical support for these predictions, and instead find evidence that exosome biogenesis and protein budding also occur at the plasma membrane.